团队介绍
动物基因编辑与模式动物团队现有固定成员5人，包括团队带头人1人，研究骨干4人，其中教授1人，副研究员2人，实验师1人，博士后1人，在读博士研究生2名、硕士研究生25名。团队近年来承担包括国家重点研发计划项目课题与子课题、转基因生物新品种培育重大专项子课题、国家自然科学基金面上项目等国家级课题6项，到账经费987万元；团队成员以第一或通讯作者在Nature Communications, Cell Research, Stem Cell Report, Frontiers in Immunology, Theriogenology等期刊发表SCI论文70余篇；获得黑龙江省自然科学一等奖等奖项2项、获得授权国家发明专利5项。该团队以基因编辑与模式动物为研究对象，主要开展如下4个方面的研究：
（1）免疫缺陷兔研究：开展SCID兔和类NOD-SCID兔的制备与应用研究，对免疫细胞发育相关基因进行编辑来获得免疫缺陷兔模型。
（2）猪体细胞核移植重编程机制的研究：以猪体细胞核移植胚胎为模型，研究体细胞重编程机制。
（3）基因编辑模型猪的生产：针对生物医学与家畜育种领域的重大需求，利用基因编辑与核移植技术生产模型猪。
（4）基因编辑技术优化和改进：围绕如何提高精确基因编辑效率、减少脱靶率，开展基因编辑机制研究。
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团队带头人 宋军
1. 个人简介
宋军，男，东北农业大学生命科学学院教授，博士生导师，动物基因编辑与模式动物团队带头人。2021年获得东北农业大学“东农学者计划”第四层次人才称号。2013年以来，一直专注于动物克隆、基因修饰与基因修饰动物模型构建的研究，以第一或通讯作者在Nature Communications, Cell Research, Stem Cell Report, Frontiers in Immunology等杂志发表SCI论文40多篇。主持国家自然科学基金面上项目1项、国家重点研发计划项目课题1项。获得黑龙江省科学技术进步奖一等奖1项（2009年）。
2. 联系方式
研究方向：动物基因编辑与应用研究
电话：18221534065
邮箱：songjun@neau.edu.cn
通讯地址：哈尔滨市香坊区长江路600号
3. 教育经历
2000.09-2004.07 东北农业大学 生物工程专业 学士
2004.09-2007.07 东北农业大学 发育生物学专业 硕士
2009.09-2013.07 中国科学院大学 生物化学与分子生物学 博士
4. 工作经历
2007.07-2009.08 中科院广州生物医药与健康与研究院 研究助理
2013.09-2018.08 美国密歇根大学医学院 博士后
2018.09-2021.03 美国密歇根大学医学院 中级研究员
2021.05-至今 东北农业大学生命科学学院 教授
5. 承担课程
本科生课程：《动物组织学与胚胎学》
研究生课程：《基因编辑与基因治疗》
6. 承担课题
1.国家重点研发计划项目：基于干细胞的多重基因修饰近交系小型猪模型培育与应用（2022YFA1105400）子课题：嵌入基因表达可调控元件的近交系工具模型培育（2022YFA1105402），2022. 12-2027. 11，597万元，主持课题二。
2.国家自然科学基金面上项目：SCID兔和类NOD-SCID兔的制备与应用（32070540），2021.01-2024.12，58万元，主持。
7. 代表论文与著作（*通讯或共同通讯标注，#共同一作标注）
Jun Song#, Mark Hoenerhoff#, Dongshan Yang, Ying Yang, Cheng Deng,
1. Luan Wen, Linyuan Ma, Brooke Pallas, Changzhi Zhao, Yui Koike, Tomonari Koike, Patrick Lester, Bo Yang, Jifeng Zhang, Y Eugene Chen, Jie Xu. Development of the nude rabbit model. Stem Cell Reports. doi.org/10.1016/j.stemcr.2021.01.010 *Contributed equally to this work.
2. Jun Song, Brooke Pallas, Dongshan Yang, Jifeng Zhang, Yash Agarwal, Y Eugene Chen, Moses Bility, Jie Xu. Immunodeficient Rabbit Models: History, Current Status and Future Perspectives. Applied Sciences. 2020, 10 (20), 7369 Invited review
3. Jun Song, Jifeng Zhang, Jie Xu, Minerva Garcia-Barrio, Y. Eugene Chen, Dongshan Yang. Genome engineering technologies in rabbits. The Journal of Biomedical Research. doi: 10.7555/JBR.34.20190133 Invited review
4. Jun Song, Guoshun Wang, Mark J. Hoenerhoff, Jinxue Ruan, Dongshan Yang, Jifeng Zhang, Jibing Yang, Patrick A. Lester, Robert Sigler, Michael Bradley, Samantha Eckley, Kelsey Cornelius, Kong Chen, Jay K. Kolls, Li Peng, Liang Ma, Yuqing Eugene Chen, Fei Sun, Jie Xu: Bacterial and Pneumocystis Infections in the Lungs of Gene-Knockout Rabbits with Severe Combined Immunodeficiency. Frontiers in Immunology, 2018 Mar 9;9:429. doi: 10.3389/fimmu.2018.00429. eCollection 2018.
5. Jun Song, Dongshan Yang, Jinxue Ruan, Jifeng Zhang, Yuqing Eugene Chen, Jie Xu: Production of immunodeficient rabbits by multiplex embryo transfer and multiplex gene targeting. Scientific Reports 2017 Sep 22;7(1):12202. doi: 10.1038/s41598-017-12201-0
6. Jun Song#, Dongshan Yang#, Jie Xu, Tianqing Zhu, Y. Eugene Chen, Jifeng Zhang: RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nature Communications 2016 Jan 28;7:10548. doi: 10.1038/ncomms10548. #Contributed equally to this work.
7. Jun Song#, Juan Zhong#, Xiaogang Guo#, Yongqiang Chen, Qingjian Zou, Jiao Huang, Xiaoping Li, Quanjun Zhang, Zhiwu Jiang, Chengcheng Tang, Huaqiang Yang, Tao Liu, Peng Li, Duanqing Pei, Liangxue Lai: Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs. Cell Research 2013 Aug;23(8):1059-62. doi: 10.1038/cr.2013.85. Epub 2013 Jul 9. #Contributed equally to this work.
ZhongHua Liu#, Jun Song#, ZhenKun Wang, JiangTian Tian, QingRan Kong, Zhong Zheng, Zhi Yin, Li Gao, HaiKun Ma, Shuang Sun, YuTian Li, HongBin Wang, R. S. Prather: Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer. Chinese Science Bulletin 03/2008;
1. 53(7):1035-1039. #Contributed equally to this work.
参与发表文章
2. Ousmane H Cissé, Liang Ma, John P Dekker, Pavel P Khil, Jung-Ho Youn, Jason M Brenchley, Robert Blair, Bapi Pahar, Magali Chabé, Koen KA Van Rompay, Rebekah Keesler, Antti Sukura, Vanessa Hirsch, Geetha Kutty, Yueqin Liu, Li Peng, Jie Chen, Jun Song, Christiane Weissenbacher-Lang, Jie Xu, Nathan S Upham, Jason E Stajich, Christina A Cuomo, Melanie T Cushion, Joseph A Kovacs. Genomic insights into the host specific adaptation of the Pneumocystis genus. Communications Biology, 4, 305 (2021). https://doi.org/10.1038/s42003-021-01799-7
3. Kyung Soo Park, Joseph D Bazzill, Sejin Son, Jutaek Nam, Seung Won Shin, Lukasz J Ochyl, Jeanne A Stuckey, Jennifer L Meagher, Louise Chang, Jun Song, David C Montefiori, Celia C LaBranche, Janet L Smith, Jie Xu, James J Moon. Lipid-based vaccine nanoparticles for induction of humoral immune responses against HIV-1 and SARS-CoV-2. Journal of Controlled Release. 2021, 330, 529-539
4. Xiubin Liang, Mohamad Bouhamdan, Xia Hou, Kezhong Zhang, Jun Song, Ke Hao, Jian-Ping Jin, Zhongyang Zhang, Jie Xu. Intestinal Dysbiosis in Young Cystic Fibrosis Rabbits. Journal of Personalized Medicine. 2021, 11 (2), 132
5. Jie Xu, Alessandra Livraghi-Butrico, Xia Hou, Carthic Rajagopalan, Jifeng Zhang, Jun Song, Hong Jiang, Hong-Guang Wei, Hui Wang, Mohamad Bouhamdan, Jinxue Ruan, Dongshan Yang, Yining Qiu, Youming Xie, Ronald Barrett, Sharon McClellan, Hongmei Mou, Qingtian Wu, Xuequn Chen, Troy D Rogers, Kristen J Wilkinson, Rodney C Gilmore, Charles R Esther Jr, Khalequz Zaman, Xiubin Liang, Michael Sobolic, Linda Hazlett, Kezhong Zhang, Raymond A Frizzell, Martina Gentzsch, Wanda K O’Neal, Barbara R Grubb, Y Eugene Chen, Richard C Boucher, Fei Sun. Phenotypes of CF rabbits generated by CRISPR/Cas9-mediated disruption of the CFTR gene. JCI insight. 2021, 6 (1)
6. Jie Xu, Jifeng Zhang, Dongshan Yang, Jun Song, Brooke Pallas, Chen Zhang, Jiafen Hu, Xuwen Peng, Neil Christensen, Renzhi Han, Y Eugene Chen. Gene Editing in Rabbits: Unique Opportunities for Translational Biomedical Research. Front. Genet. 12:642444. doi: 10.3389/fgene.2021.642444
7. Tomonari Koike, Yui Koike, Dongshan Yang, Yanhong Guo, Oren Rom, Jun Song, Jie Xu, Yajie Chen, Yanli Wang, Tianqing Zhu, Minerva T Garcia-Barrio, Jianglin Fan, Y Eugene Chen, Jifeng Zhang. Human apolipoprotein A-II reduces atherosclerosis in knock-in rabbits. Atherosclerosis. 2021, 316, 32-40
Oren Rom, Yuhao Liu, Zhipeng Liu, Ying Zhao, Jianfeng Wu, Alia Ghrayeb, Luis Villacorta, Yanbo Fan, Lin Chang, Lu Wang, Cai Liu, Dongshan Yang,
1. Jun Song, Jason C Rech, Yanhong Guo, Huilun Wang, Guizhen Zhao, Wenying Liang, Yui Koike, Haocheng Lu, Tomonari Koike, Tony Hayek, Subramaniam Pennathur, Chuanwu Xi, Bo Wen, Duxin Sun, Minerva T Garcia-Barrio, Michael Aviram, Eyal Gottlieb, Inbal Mor, Wanqing Liu, Jifeng Zhang, Y Eugene Chen. Glycine-based treatment ameliorates NAFLD by modulating fatty acid oxidation, glutathione synthesis, and the gut microbiome. Science Translational Medicine. 2021, 12 (572)
2. Linyuan Ma, Jinxue Ruan, Jun Song, Luan Wen, Dongshan Yang, Jiangyang Zhao, Xiaofeng Xia, Y Eugene Chen, Jifeng Zhang, Jie Xu. MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Nature communications. 2020, 11 (1), 1-11
3. Ali Z Siddiqui, Umar F Bhatti, Qiufang Deng, Ben E Biesterveld, Yuzi Tian, Zhenyu Wu, Julia Dahl, Baoling Liu, Jie Xu, Yui Koike, Jun Song, Jifeng Zhang, Yongqing Li, Hasan B Alam, Aaron M Williams. Cl-Amidine Improves Survival and Attenuates Kidney Injury in a Rabbit Model of Endotoxic Shock. Surgical Infections. ahead of printhttp://doi.org/10.1089/sur.2020.189
4. Liang Ma, Zehua Chen, Ousmane H Cissé, Jamie L Rothenburger, Alice Latinne, Lisa Bishop, Robert Blair, Jason M Brenchley, Magali Chabé, Xilong Deng, Vanessa Hirsch, Rebekah Keesler, Geetha Kutty, Yueqin Liu, Daniel Margolis, Serge Morand, Bapi Pahar, Li Peng, Koen KA Van Rompay, Xiaohong Song, Jun Song, Antti Sukura, Sabrina Thapar, Honghui Wang, Christiane Weissenbacher-Lang, Jie Xu, Chao-Hung Lee, Claire Jardine, Richard A Lempicki, Melanie T Cushion, Christina A Cuomo, Joseph A Kovacs: Diversity and Complexity of the Large Surface Protein Family in the Compacted Genomes of Multiple Pneumocystis Species, mBio 2020. 11 (2). DOI: 10.1128/mBio. 02878-19
5. Luan Wen*, Changzhi Zhao*, Jun Song, Linyuan Ma, Jinxue Ruan, Xiaofeng Xia, Y Eugene Chen, Jifeng Zhang, Peter X Ma, Jie Xu: CRISPR/Cas9-Mediated TERT Disruption in Cancer Cells. International Journal of Molecular Sciences. 2020, 21(2), 653; https://doi.org/10.3390/ijms21020653
6. SM Ballou, J Song, J Xu, PA Lester. Husbandry Efforts for Housing and Maintaining Immunodeficient Transgenic Rabbits. Laboratory Animal Science Professional, 2019, 7 (3), 58-59
7. Linyuan Ma*, Lydia Jang*, Jian Chen, Jun Song, Dongshan Yang, Jifeng Zhang, Y. Eugene Chen, Jie Xu: CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation. Journal of Visualized Experiments. 2019. (148), e59512, doi:10.3791/59512 .
Jifeng Zhang, Manabu Niimi, Dongshan Yang, Jingyan Liang, Jie Xu, Tokuhide Kimura, Anna V. Mattew, Yanhong Guo, Yanbo Fan, Tianqing Zhu, Jun Song, Rose Ackermann, Yui Koike, Anna Schwendeman, Liangxue Lai, Subramaniam Pennathur, Minerva Garcia-Barrio, Jianglin Fan, Y. Eugene
1. Chen: Deficiency of Cholesteryl Ester Transfer Protein Protects Against Atherosclerosis in Rabbits. Arteriosclerosis Thrombosis and Vascular Biology, 2017 Jun;37(6):1068-1075. doi: 10.1161/ATVBAHA.117.309114.
2. Zhen Wang, Jifeng Zhang, Hong Li, Junyi Li, Manabu Niimi, Guohui Ding, Haifeng Chen, Jie Xu, Hongjiu Zhang, Ze Xu, Yulin Dai, Tuantuan Gui, Shengdi Li, Zhi Liu, Sujuan Wu, Mushui Cao, Lu Zhou, Xingyu Lu, Junxia Wang, Jing Yang, Yunhe Fu, Dongshan Yang, Jun Song, Tianqing Zhu, Shen Li, Bo Ning, Ziyun Wang, Tomonari Koike, Masashi Shiomi, Enqi Liu, Luonan Chen, Jianglin Fan, Y. Eugene Chen, Yixue Li: Hyperlipidemia-associated gene variations and expression patterns revealed by whole-genome and transcriptome sequencing of rabbit models. Scientific Reports 2016 Jun 1;6:26942. doi: 10.1038/srep26942.
3. Dongshan Yang, Jun Song, Jifeng Zhang, Jie Xu, Tianqing Zhu, Zhong Wang, Liangxue Lai, Y. Eugene Chen: Identification and characterization of rabbit ROSA26 for gene knock-in and stable reporter gene expression. Scientific Reports 2016 Apr 27;6:25161. doi: 10.1038/srep25161.
4. Longquan Quan, Yongqiang Chen, Jun Song, Quanmei Yan, Quanjun Zhang, Sisi Lai, Nana Fan, Jige Xin, Qingjian Zou, Liangxue Lai: Establishment of a Rabbit Oct4 Promoter-Based EGFP Reporter System. PLoS ONE 2014 Oct 31;9(10):e109728. doi: 10.1371/journal.pone.0109728. eCollection 2014
5. Jiao Huang, Xiaogang Guo, Nana Fan, Jun Song, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Quanmei Yan, Xiaoling Yi, Axel Schambach, Jon Frampton, Miguel A Esteban, Dongshan Yang, Huaqiang Yang, Liangxue Lai: RAG1/2 Knockout Pigs with Severe Combined Immunodeficiency. The Journal of Immunology 2014 Aug 1;193(3):1496-503. doi: 10.4049/jimmunol.1400915
6. Xiaoping Li, Yi Yang, Lei Bu, Xiaogang Guo, Chengcheng Tang, Jun Song, Nana Fan, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Xiaoling Yi, Longquan Quan, Songcai Liu, Zhenguo Yang, Hongsheng Ouyang, Y Eugene Chen, Zhong Wang, Liangxue Lai: Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing. Cell Research 2014 Apr;24(4):501-4. doi: 10.1038/cr.2014.15.
7. Yu Wang, Nana Fan, Jun Song, Juan Zhong, Xiaogang Guo, Weihua Tian, Quanjun Zhang, Fenggong Cui, Li Li, Philip N Newsome, Jon Frampton, Miguel A Esteban, Liangxue Lai: Generation of knockout rabbits using transcription activator-like effector nucleases. Cell regeneration 2014 Feb 5;3(1):3. doi: 10.1186/2045-9769-3-3. eCollection 2014.
8. Jige Xin, Huaqiang Yang, Nana Fan, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Xiaoping Li, Jun Song, Yi Yang, Qingjian Zou, Quanmei Yan, Yangzhi Zeng, Liangxue Lai: Highly efficient generation of GGTA1 biallelic knockout inbred mini-pigs with TALENs. PLoS ONE 2013 Dec 17;8(12):e84250. doi: 10.1371/journal.pone.0084250. eCollection 2013.
1. Jiangtian Tian, Jun Song, Haibin Li, Dongshan Yang, Xiaoping Li, Hongsheng Ouyang, Liangxue Lai: Effect of Donor Cell Type on Nuclear Remodelling in Rabbit Somatic Cell Nuclear Transfer Embryos. Reproduction in Domestic Animals 2012 Aug;47(4):544-52. doi: 10.1111/j.1439-0531.2011.01915.x. Epub 2011 Oct 29
2. Dongshan Yang, Huaqiang Yang, Wei Li, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Nana Fan, Jun Song, Jiangtian Tian, Feng Li, Jifeng Zhang, Lin Chang, Duanqing Pei, Y Eugene Chen, Liangxue Lai: Generation of PPARgamma mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning. Cell Res. Cell Research 2011 Jun;21(6):979-82. doi: 10.1038/cr.2011.70. Epub 2011 Apr 19.
3. Wei Deng, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Jun Song, Nana Fan, Zhaoming Liu, Yu Zhao, Qinghong Wu, Bayaer Nashun, Jiangjing Tang, Zhenfang Wu, Weiwang Gu, Liangxue Lai: Use of the 2A Peptide for Generation of Multi-Transgenic Pigs through a Single Round of Nuclear Transfer. PLoS ONE 2011;6(5):e19986. doi: 10.1371/journal.pone.0019986. Epub 2011 May 13.
4. Bou Gerelchimeg, Liu Li-Qing, Zheng Zhong, Tian Jiang-Tian, Kong Qing-Ran, Song Jun, Wang Xue-Dong, Liu Zhong-Hua: Effect of chilling on porcine germinal vesicle stage oocytes at the subcellular level. Cryobiology 2009 Aug;59(1):54-8. doi: 10.1016/j.cryobiol.2009.04.005. Epub 2009 Apr 24.
8. 获得奖励
东北民猪体细胞核移植及绿色荧光蛋白转基因猪研究，黑龙江省科学技术进步一等奖，5/8，2009年。
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团队成员 牟彦双
1. 个人简介
牟彦双，男，东北农业大学生命科学学院副研究员，硕士研究生导师。2015年获得东北农业大学“青年才俊”人才称号。多年以来，一直专注于哺乳动物基因编辑的研究，以第一或通讯作者在Theriogenology ,FEBS Letter等杂志发表SCI论文30多篇。主持国家自然科学基金等课题5项。获得黑龙江省自认科学进步一等奖1项（2009年）。
2. 联系方式
研究方向：哺乳动物基因编辑技术研究
电话：0451-55190413
邮箱：muyanshuang@126.com
通讯地址：哈尔滨市香坊区长江路600号
3. 教育经历
1999.09-2003.07 东北农业大学 生物科学专业 学士
2003.09-2008.01 东北农业大学 动物遗传育种与繁殖专业 博士
4. 工作经历
2008.07-2011.06 东北农业大学 博士后
2012.01-2013.12 日本熊本大学 访问学者
2014.09-至今 东北农业大学 副研究员
5. 承担课程
本科生课程：《动物组织学与胚胎学》
研究生课程：《发育生物学前沿专题讲座》
6. 承担课题
1、黑龙江省自然科学基金联合项目：IGFBP3基因调控猪脂肪细胞增殖分化机制研究（LH2020C016），2020.07-2023.07，10万元，主持。
2、农业部转基因生物新品种培育重大专项课题子任务：动物重要基因克隆及功能验证（2016ZX08009003-006），2016.01-2020.12，134万元，主持。
3、东北农业大学“青年才俊”课题：基于CRISPR/Cas9技术筛选猪成肌细胞增殖分化关键基因（14QC06），2015.01-2016.12，6万元，主持。
4、哈尔滨医科大学心肌缺血省部共建重点实验室开放课题：游离型载体在小鼠心脏的表达研究（KF201320），2014.01-2016.12，4万元，主持。
5、国家自然科学基金青年项目基金，精子介导piggyBac转座子高效制备转基因动物机理研究（31101033），2012.01-2014.12，21万元，主持。
7. 代表论文与著作（*通讯或共同通讯标注，#共同一作标注）
1. Z. Yina XZ, J. Lia, Y. Jiaoa, Q. Konga,and Y. Mu*: Identification of Imprinted Genes and Their Differentially Methylated Regions in Porcine. Russ J Genet 2019.
2. Yanshuang Mu#, Li Leng, Zhiping Cao, Yumao Li, Hui Li and Yuxiang Wang: Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes. Journal of Poultry Science 2019, 56:177-185.
3. Li XY, Yu TF, Li M, Wang YQ, Meng B, Mu Y*: NANOG improves type I collagen expression in human fetal scleral fibroblasts. Arch Biol Sci 2019, 71:63-70.
4. Kong QR, Zhang JM, Zhang XL, Zong M, Zheng KL, Liu L, Meng FX, Zhang TT, Weng XG, Mu YS*, Liu ZH*: Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine oocyte. Theriogenology 2019, 135:19-24.
5. Kong Q, Quan X, Du J, Tai Y, Liu W, Zhang J, Zhang X, Mu Y*, Liu Z*: Endo-siRNAs regulate early embryonic development by inhibiting transcription of long terminal repeat sequence in pig. Biol Reprod 2019.
6. Yu TF, Li M, Dong HY, Xie PY, Sun WZ, Meng B, Mu YS*: The effect of knockdown of insulin receptor substrates 1 and 2 on glucose and lipid metabolism in human hepatoblastoma cells. Arch Biol Sci 2018, 70:757-764.
7. Mu Y#, Jiao M, Zhao Y, Lv J, Wang J, Hao J, Zhang X, Kong Q, Liu Z: A method for tracing exogenous DNA uptake in live spermatozoa and embryos. Pol J Vet Sci 2018, 21:193-202.
8. Mu Y#, Jiang C, Jiao M, Zhao Y, Lv J, Liu Z: Effect of the acrosome reaction on the efficiency of sperm-mediated DNA transfer. Pol J Vet Sci 2018, 21:755-762.
9. Hai T#, Cao CW#, Shang HT#, Guo WW#, Mu YS#, Yang SLL, Zhang Y, Zheng QT, Zhang T, Wang XL, et al: Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife 2017, 6.
10. Mu Y#, Jin S, Shen J, Sugano A, Takaoka Y, Qiang L, Imbimbo BP, Yamamura K, Li Z: CHF5074 (CSP-1103) stabilizes human
1. Z. Yina XZ, J. Lia, Y. Jiaoa, Q. Konga,and Y. Mu*: Identification of Imprinted Genes and Their Differentially Methylated Regions in Porcine. Russ J Genet 2019.
2. Yanshuang Mu#, Li Leng, Zhiping Cao, Yumao Li, Hui Li and Yuxiang Wang: Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes. Journal of Poultry Science 2019, 56:177-185.
3. Li XY, Yu TF, Li M, Wang YQ, Meng B, Mu Y*: NANOG improves type I collagen expression in human fetal scleral fibroblasts. Arch Biol Sci 2019, 71:63-70.
4. Kong QR, Zhang JM, Zhang XL, Zong M, Zheng KL, Liu L, Meng FX, Zhang TT, Weng XG, Mu YS*, Liu ZH*: Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine oocyte. Theriogenology 2019, 135:19-24.
5. Kong Q, Quan X, Du J, Tai Y, Liu W, Zhang J, Zhang X, Mu Y*, Liu Z*: Endo-siRNAs regulate early embryonic development by inhibiting transcription of long terminal repeat sequence in pig. Biol Reprod 2019.
6. Yu TF, Li M, Dong HY, Xie PY, Sun WZ, Meng B, Mu YS*: The effect of knockdown of insulin receptor substrates 1 and 2 on glucose and lipid metabolism in human hepatoblastoma cells. Arch Biol Sci 2018, 70:757-764.
7. Mu Y#, Jiao M, Zhao Y, Lv J, Wang J, Hao J, Zhang X, Kong Q, Liu Z: A method for tracing exogenous DNA uptake in live spermatozoa and embryos. Pol J Vet Sci 2018, 21:193-202.
8. Mu Y#, Jiang C, Jiao M, Zhao Y, Lv J, Liu Z: Effect of the acrosome reaction on the efficiency of sperm-mediated DNA transfer. Pol J Vet Sci 2018, 21:755-762.
9. Hai T#, Cao CW#, Shang HT#, Guo WW#, Mu YS#, Yang SLL, Zhang Y, Zheng QT, Zhang T, Wang XL, et al: Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife 2017, 6.
10. Mu Y#, Jin S, Shen J, Sugano A, Takaoka Y, Qiang L, Imbimbo BP, Yamamura K, Li Z: CHF5074 (CSP-1103) stabilizes human transthyretin in mice humanized at the transthyretin and retinol-binding protein loci. FEBS Lett 2015, 589:849-856.
11. Kong QR, Hai T, Ma J, Huang TQ, Jiang DD, Xie BT, Wu ML, Wang JQ, Song YR, Wang Y, Mu YS* and Liu ZH*: Rosa26 Locus Supports Tissue-Specific Promoter Driving Transgene Expression Specifically in Pig. PLoS One 2014, 9.
12. 王玥，牟彦双*，刘忠华: 基于CRISPR_Cas系统的单碱基编辑技术研究进展. 中国生物工程杂志 2020, 40:58-66.
《转基因动物概述》, 东北林业大学出版社, 2011年, 主编。
8. 获得奖励
东北民猪体细胞核移植及绿色荧光蛋白转基因猪研究，黑龙江省科技奖自然科学类一等奖，第4名，2009年。
9. 专利和标准
牟彦双，刘忠华，李佳男等。一种附加型CRISPR/Cas9表达载体及其
构建方法与应用。专利授权号：ZL201910212739.1，中国，2022年。
翁晓刚，牟彦双，张宇霆等，一种猪冻精解冻稀释液及其配置方法，ZL2017108028371，中国，2018年。
刘忠华，郇延军，牟彦双等，一种提高体外受精胚胎发育率的方法，ZL2013106081582，中国，2018年。
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团队成员 翁晓刚
1. 个人简介
翁晓刚，男，东北农业大学生命科学学院副研究员，博士生导师。主要研究方向为猪体细胞核移植重编程机制，以及利用基因编辑与核移植技术生产模型猪。在Theriogenology、Reprod Domest Anim、J Anim Sci等杂志发表SCI论文10余篇。主持国家重点研发计划项目子课题、国家自然科学基金面上项目、国家自然科学基金青年科学基金等项目8项。获得黑龙江省高校科学技术一等奖（2021年）。获得黑龙江省高校青年创新人才、东北农业大学“青年才俊”人才称号。
2. 联系方式
研究方向：猪体细胞核移植重编程机制与应用
邮箱：wengxg@neau.edu.cn
通讯地址：哈尔滨市香坊区长江路600号
3. 教育经历
2005. 09-2009. 07 山东农业大学 动物医学专业 学士
2009. 09-2014. 07 中国农业大学 临床兽医学专业 硕博连读
4. 工作经历
2014 -2020 东北农业大学 助理研究员
2020-至今 东北农业大学 副研究员
5. 承担课程
本科生课程：《实验动物学》、《动物胚胎工程》
研究生课程：《胚胎工程在育种及生物医药中的应用》
6. 承担课题
国家重点研发计划项目子课题：
（1）家畜干细胞育种关键技术研究及工程化组装，2022.8-2027.7，100万元，主持。
（2）猪初始态（naive）多能干细胞系建立及多能性调控机制解析，2016.1-2020.12，2833万元，参与。
国家自然科学基金项目：
（1）猪体细胞核移植胚胎X染色体活性变化及其调控机制探索2021.1-2024.12，58万元，主持。
（2）H3K9me3与H3K27me3对猪体细胞重编程影响机制，2017.1-2019.12，19万元，主持。
黑龙江省自然基金面上项目：
猪体细胞核移植胚胎H3K27me3异常重编程机制, 2017.10-2020.10，6万元，主持。
7. 代表论文与著作（*通讯或共同通讯标注，#共同一作标注）
1. Weng XG#, Cai MM#, Zhang YT, Liu Y, Liu C, Liu ZH. Improvement in the in vitro development of cloned pig embryos after kdm4a overexpression and an H3K9me3 methyltransferase inhibitor treatment. Theriogenology. 2020, 146:162-170.
2. Weng XG#, Cai MM#, Zhang YT, Liu Y, Gao ZL, Song J, Liu ZH. Effect of Astragalus polysaccharide addition to thawed boar sperm on in vitro fertilization and embryo development. Theriogenology. 2018; 121:21-26.
3. Weng XG#, Liu Y#, Zhou SH, Zhang YT, Shao YT, Xu QQ, Liu ZH. Evaluation of porcine circovirus type 2 infection in in vitro embryo production using naturally infected oocytes. Theriogenology. 2019; 126:75-80.
4. Zhang YT, Liu Y, Liang HL, Xu QQ, Liu ZH*, Weng XG*. Metabolomic differences of seminal plasma between boars with high and low average conception rates after artificial insemination. Reproduction in Domestic Animals. 2021; 56:161-171.
5. Zhang YT, Yao W, Chai MJ, Liu WJ, Liu Y, Liu ZH*, Weng XG*. Evaluation of porcine urine-derived cells as nuclei donor for somatic cell nuclear transfer. Journal of Veterinary Science. 2022, 23:40-53.
Zhang YT, Liang HL, Liu Y, Zhao M, Xu QQ, Liu ZH*, Weng XG*: Metabolomic Analysis and Identification of Sperm Freezability-Related Metabolites in Boar Seminal Plasma. Animals.
1. 2021; 11:1939.
2. Xie BT, Zhang H, Wei RY, Li QN, Weng XG, Kong QR, Liu ZH. Histone H3 lysine 27 trimethylation acts as an epigenetic barrier in porcine nuclear reprogramming. Reproduction. 2016; 151:9-16.
3. Bou G, Liu SC, Sun MJ, Zhu J, Xue BH, Guo J, Zhao YM, Qu B, Weng XG, Wei YC. CDX2 is essential for cell proliferation and polarity in porcine blastocysts. Development. 2017; 144:1296-1306.
4. Liu SC, Bou GC, Zhao JC, Guo SM, Guo J, Weng XG, Yin Z, Liu ZH. Asynchronous CDX2 expression and polarization of porcine trophoblast cells reflects a species‐specific trophoderm lineage determination progress model. Molecular Reproduction and Development. 2018; 85:590-598.
5. Kong QR, Yang X, Zhang H, Liu SC, Zhao JC, Zhang JM, Weng XG, Jin JX, Liu ZH. Lineage specification and pluripotency revealed by transcriptome analysis from oocyte to blastocyst in pig. The FASEB Journal. 2020; 34:691-705.
8. 获得奖励
早期胚胎分化与多能性分子调控机制，黑龙江省高校科学技术一等奖，第四名，2021年。
9. 专利和标准
翁晓刚，刘忠华等，一种猪冻精解冻稀释液及其配制方法. 专利授权号：ZL201710802837.1，中国，2018年
刘忠华，翁晓刚等，一种诱导后备母猪同期发情的方法. 专利授权号：ZL201810813305.2，中国，2021年
---------------------------------------------------------------------------------------------------------------团队成员 徐倩倩
1. 个人简介
徐倩倩 ，女，东北农业大学生命科学学院实验师。2018年以来，一直专注于猪胚胎上的显微操作技术。
2. 联系方式
研究方向：猪胚胎显微操作技术
电话：13936093212
邮箱：xuqianqian@neau.edu.cn
通讯地址：哈尔滨市香坊区长江路600号
3. 教育经历
2008. 09-2012. 06 东北农业大学 动物科学技术专业 学士
2012. 09-2015. 06 东北农业大学 动物营养与饲料科学专业 硕士
4. 工作经历
2015. 07-2018. 07 哈尔滨国生生物科技股份有限公司 技术员
2018. 09-至今 东北农业大学 实验员
5. 承担课程
本科生课程：无
研究生课程：无
6. 承担课题
（1）国家自然基金委员会，面上项目，31970588，大鼠-小鼠异种杂合二倍体胚胎干细胞中异源基因组的互作模式的研究，2020.1-2023.12，58万，在研，参加
（2）国家自然基金委员会，面上项目，猪体细胞核移植胚胎X染色体活性变化及其调控机制探索，2021.1-2024.12，58万元，在研，参加。
（3）国家自然科学基金，面上项目，32070540，SCID兔和类NOD-SCID兔的制备与应用，2021.1-2024.12，58万元，在研，参加。
（4）黑龙江省自然科学基金，重点项目，ZD2022C004，猪高效基因编辑体系创建及应用研究，2022.07-2025.07,50万，在研，参加
7. 代表论文与著作（*通讯或共同通讯标注，#共同一作标注）
1. Xu Qianqian#, Sun Junfeng#, Gao Mengying, Zhao Shasha, Liu Huairan, Zhang TingTing, Han Zongxi, Kong Xiangang, Liu Shengwang. Genetic, antigenic, and pathogenic characteristics of Newcastle disease viruses isolated from geese in Chinas. Journal of Veterinary Diagnostic Investigation, 2017;29(4):489-498.
2. Xu Qianqian, Chen Yuqiu, Zhao Wenjun, Zhang Tingting, Liu Chenggang, Qi Tianming, Han Zongxi, Shao Yuhao, Ma Deying*, Liu Shengwang *. Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage. Frontiers in Microbiology, 2016; 7:1587.
3. Xu Qianqian, Han Zongxi, Wang Qiuling, Zhang Tingting , Gao Mengying, Zhao Yan, Shao
Yuhao, Li Huixin, Kong Xiangang, Liu Shengwang. Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks. Avian Pathology, 2016;45(1):54-65.
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团队成员 张宇霆
1. 个人简介
张宇霆，男，在站博士后，博士毕业于东北农业大学生命科学学院发育生物学专业。主要从事猪精液冷冻保存以及体细胞核移植重编程的表观修饰障碍等研究，以第一作者在Reprod Domest Anim、Animals、J Vet Sci杂志发表SCI论文3篇，已获得授权专利2项，于2021年获得黑龙江省博士后基金资助（一等）。
2. 联系方式
研究方向：猪精液冷冻保存以及体细胞核移植重编程的表观修饰障碍
邮箱：zhangyuting217@neau.edu.cn
通讯地址：哈尔滨市香坊区长江路600号
3. 教育经历
2010. 09-2014. 07 大庆师范学院 生物科学专业 学士
2014. 09-2017. 07 东北农业大学 发育生物学专业 硕士
2017. 09-2021. 07 东北农业大学 发育生物学专业 博士
4. 工作经历
2021 -至今 东北农业大学 博士后
5. 承担课程
无
6. 承担课题
黑龙江省博士后资助（一等）：
猪精子耐冻性提升与精浆耐冻性生物标志物验证, 2021. 11月，10万元。
7. 代表论文与著作（*通讯或共同通讯标注，#共同一作标注）
1. Zhang YT, Liu Y, Liang HL, Xu QQ, Liu ZH*, Weng XG*. Metabolomic differences of seminal plasma between boars with high and low average conception rates after artificial insemination. Reproduction in Domestic Animals. 2021; 56:161-171.
2. Zhang YT, Yao W, Chai MJ, Liu WJ, Liu Y, Liu ZH*, Weng XG*. Evaluation of porcine urine-derived cells as nuclei donor for somatic cell nuclear transfer. Journal of Veterinary Science. 2022, 23:40-53.
3. Zhang YT, Liang HL, Liu Y, Zhao M, Xu QQ, Liu ZH*, Weng XG*: Metabolomic Analysis and Identification of Sperm Freezability-Related Metabolites in Boar Seminal Plasma. Animals. 2021; 11:1939.
Weng XG, Cai MM, Zhang YT, Liu Y, Liu C, Liu ZH. Improvement in the in vitro development of cloned pig embryos after kdm4a
1. overexpression and an H3K9me3 methyltransferase inhibitor treatment. Theriogenology. 2020, 146:162-170.
2. Weng XG, Cai MM, Zhang YT, Liu Y, Gao ZL, Song J, Liu ZH. Effect of Astragalus polysaccharide addition to thawed boar sperm on in vitro fertilization and embryo development. Theriogenology. 2018; 121:21-26.
3. Weng XG, Liu Y, Zhou SH, Zhang YT, Shao YT, Xu QQ, Liu ZH. Evaluation of porcine circovirus type 2 infection in in vitro embryo production using naturally infected oocytes. Theriogenology. 2019; 126:75-80.
8. 获得奖励
无
9. 专利和标准
翁晓刚，牟彦双，张宇霆等，一种猪冻精解冻稀释液及其配制方法. 专利授权号：ZL201710802837.1，中国，2018年
刘忠华，翁晓刚，张宇霆等，一种诱导后备母猪同期发情的方法. 专利授权号：ZL201810813305.2，中国，2021年
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